A “2＋2 days” Fast Protocol for the Generation of Dendritic
Cells from Human Blood Monocytes

A “2＋2 days” fast protocol for the generation of dendritic cells（DC） from high purity human monocytes in vitro has been established. During the 2-step differentiation and activation process, we demonstrated that 2 days of culture with GM-CSF and IL-4 were sufficient to generate immature DCs capable of antigen uptake. Similarly the mature DCs were activated with a cocktail of rTNF-α, IL-1β, IL-6, and PGE₂ from immature DC in 2 days. This “2＋2” fast DC had the same phenotype and function as the “6＋2” standard DC.