Studies on the Structure and Function of Amphioxus Ribosomes Ⅰ.Isolation of Amphioxus tRNAs and 5S RNAs

This article reports the method for preparing Trna and 5S RNA from amphioxus (Branchiostoma belcheri Gray).Whole tissues of frozen amphioxus were homogenized in buffer Ⅰ with a Waring blendor at 0 ℃ for 30s,then the lowmolecular-weight RNAs were extracted by the phenol method.By means of column chromatography of DEAE-celloluse,DEAE-Sephadex A-50 and Sephadex G-100,tRNAs and 5S RNAs were obtained separately.They were further purified by electrophoresis on polyacrylamide gel.The accepting activity of amphioxus tRNA was determined:glycine,6.3%,alanine,5.2% and tyrosine,21%-25%.