A New Technique For Chromasome Preparation by Hypotonising The Intact Bone Marrow

We describe a new feasible chromosome procedure detailed as follows:1.Take out of the femur or tibia from birds and small mammals,and sternum or rib from large mammals.2.Scissor it carefully and treat for 50 minutes with hypotonic solution.3.Squeeze it,and fix with Carnoys solution.4.Prepare the slide by air-drying method.We have obtained quite a few good chromosome specimens by this procedure.About 66% of the birds and almost all of the mammals chromosome prepartions are succeed in our experiments by this technique.Using this method we can begin cytogenetic works in some poor conditions e.g.when centrifuge or electricity is not available.This technique may be employed in some special research programs as well as in field investigations.