Studies on Electron Microscopic Enzymo-Cytochemistry of Organelles of Resting Cyst in Paraurostyla weissei (Ciliophora,Hypotrichida)

The study on the organelles and their functions in resting Paraurostyla weissei was carried out with electron microscopic enzymo-cytochemistry method.The results revealed:1)the activity of acid phosphatase (ACPase) was confined to the autophagic vacuoles of the different stages and the enzyme reaction particles wene made up the vacuolar bodies of various shapes;2)the activity of adenosine triposphatase (ATPase) also presented in the autophagic vacuoles,and the enzyme reaction particles formed the vacuolar or spherical bodies of various sizes;3)the localization of succinic dehydrogenase activity was in a few of the mitochondria separately scattered in the cytoplasm and there were a small number of the enzyme reaction granules in the mitochondrial inner membrane ranged with the out membrane although no reaction had been seen at a lot of the mitochondria converged to each other.Thus it was supposed from the above results:1)the digestion within autophagic vacuoles that was confirmed by the ACPase reaction might be a main life process which made use of the material and supplies the energy requirement for the resting cell in a physiologically special state;2)the ATPase reaction was at the autophagic vacuole,that was,the lysoome,in which the digestive duration was bound up with the energy consumption;3)The mitochondria that were mostly converged to each other might be the nonfunctional organelles in the resting cell while a few of those separately scattered in the cytoplasm might be the functional organelles.