Genetic Diversity of Blood Protein Among Main Indigenous Pig Breeds in China

A total of 201 blood samples including 9 indigenous pig breeds (Sus scrofa domesticus) in China and Yorkshine pigs were collected in order to evaluate the genetic diversity among Chinese pig breeds.Thirty blood protein loci were studied by horizontal starch gel electrophoresis,among which 6 loci (MDH,PEPB,AK,6PGD,ADA,PHI) were found to be polymorphic.The percentage of polymorphic loci (P),the mean individual heterozygosity (H) and the mean number of alleles (A) are 0.200,0.065 and 1.300,respectively.The degree of genetic variability of Chinese indigenous pig breeds as a whole was higher than that of goats,lower than those of cattle,horses and sheep.Using gene frequencies of the 30 loci,the pairwise genetic distance among the 9 indigenous breeds in China were calculated by software PHYLIP3.5 c.The phylogenetic trees were constructed using NJ and UPGMA methods.The results suggest that the geographical distribution do not consist with the genetic relationship among Chinese indigenous pig breeds.The genetic diversity among Chinese indigenous pig breeds is low.