Construction of a Genetic Linkage Map in Fenneropenaeus chinensis Using RAPD and SSR Markers
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Abstract
The primary genetic linkage maps of Fenneropenaeus chinensis were constructed by using a "two-way pseudo-testcross" strategy with RAPD and SSR markers. Parents and F1 progeny were used as segregating populations. Sixty-one RAPD primers and 20 pairs of SSR primers were screened from 460 RAPD primers and 44 pairs of SSR primers. These primers were used to analyze the parents and 82 progeny of the mapping family. One hundred and forty-six marker primers (128 RAPDs, 18 microsatellites) were used in the female and 127 primers (109 RAPDs, 18 microsatellites) in the male were used as segregating markers. The female linkage map included eight linkage groups, nine triplets and 14 doublets, spanning 1 173 cM with the average marker density of 11.28 cM. The observed coverage was 59.36%. The male linkage map included 10 linkage groups, 12 triplets and seven doublets, spanning 1 144.6 cM with the average marker density of 12.05 cM. The observed coverage was 62.01%. The construction of the F. chinensis genetic linkage maps here open a new prospect for marker-assisted selection programs, comparative genomics and quantitative trait loci (QTL) gene location and cloning.
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