Studies on the Thrombin-Like Principles of Agkistrodon acutus Venom.Ⅱ.Studies on Activity of Enzymology and Hemotology

Four kinds of thrombin-like principles (TLP) for comparing their enzymological properties were studied.In vitro,the result shows that TLP4>TLP3>TLP1>TLP2 for the activity of coagulation and the activity of arginineesterase is higher in TLP1 and TLP2 than that in TLP3 and TLP4.Defibrination of TLP1 or TLP2 is strong,but hemocoagulation of TLP4 and TLP3 is strong in vivo.The four enzymes show differences in influence of Ca,pH,EDTA,temperature and heparin upon the activities of the enzymes.The result is similar to the thrombin-like enzymes obtained from Bothrops asper venom.The rerult may make sense in their application.