Mitochondrial DNA Control Region Structure and MolecularPhylogenetic Relationship of Carangidae
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Abstract
Complete sequences of the mitochondrial DNA control region from nine species of Carangidae were amplified by Polymerase Chain Reaction (PCR), and were aligned by Clustal W with three other species of Carangidae from GenBank. According to the alignment, three domains, the termination associated sequence domain (TAS), the central conserved domain (CD) and the conserved sequence block domain (CSB), were identified in the mtDNA control region of Carangidae. A termination associated sequence (TACAT) and its reverse complementary sequence (ATGTA) were found in the TAS domain. Three conserved blocks (CSB-F, CSB-E,CSB-D) in the CD domain and three conserved sequence blocks (CSB-1, CSB-2, CSB-3) in the CSB domain were also identified. Repeat sequences were found in the 5′ and 3′ ends of the control region in Scomberoides commersonianus. With Lates calcarifer as the outgroup, the molecular phylogenetic relationship of Carangidae was analyzed using the neighbor-joining (NJ) method in PAUP 4.0b 10. The results showed that Carangidae could be divided into four subfamilies: Caranginae, Seriolinae, Trachinotinae and Chorineminae. Each of these subfamilies formed a monophyly. Caranginae and Seriolinae formed a sister group, and Trachinotini was sister to the clade of Caranginae and Seriolinae. Chorineminae, which located in the base of the tree and was sister to the other three subfamilies.
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