Detection of Mamu-DRB*W101 and Mamu-DRB*W201 in Chinese Rhesus Monkeys by PCR-SSP
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Abstract
The aim of this study was to obtain preliminary data about the frequency of 2 MHC class II genes, Mamu-DRB*W101 and Mamu-DRB*W201 among Chinese rhesus macaques. Blood was obtained, and DNA was extracted using QIAamp DNA Blood Mini Kit according to the manufacturer’s instruction. PCR was performed using sequence specific primers to amplify the 2nd exon of Mamu-DRB*W101 and Mamu-DRB*W201. The nucleotide sequences of the amplicons were confirmed by sequencing. In the 136 samples tested in this study, 10 samples were found positive by PCR for Mamu-DRB*W101 and 10 samples positive for Mamu-DRB*W201. The frequency of positive individuals among the Chinese macaques tested was 7.35% for both Mamu-DRB*W101 and Mamu-DRB*W201. The nucleotide sequences of the amplicons were identical to the published sequences. This study demonstrated the existence of both Mamu-DRB*W101 and Mamu-DRB*W201 among Chinese rhesus macaques and provided the basis for large scale typing of these two alleles and studies using Chinese rhesus macaques in SAIDS.
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