Cloning and Sequence Analysis of cDNAs Encoding Four C-type Lectin-like Proteins from Snake Trimeresurus mucrosquamatus

The total mRNA was prepared and purified from the venom glands of snake Trimer esurus mucrosquamatus.The cDNAs encoding C-type lectin-like proteins were amplified by RT-PCR and cloned into the PMD 18-T vectors.The positive clones encoding four C-type lectin-like proteins (TML-1,TML-2,TML-3 and TML-4) were selected and sequenced.Amino acid sequences of these proteins were deduced and each contains carbohydraterecognition domain (CRD).Homology comparison and cystein position analysis indicate that TML-1 and TML-2 might be the α and β subunits of a C-type lectin-like protein similar to flavocetin-A,TML-3 the α subunit of a C-type lectin-like protein similar to GPIb-bp,and TML-4 the β subunit of a C-type lectin-like protein similar to Ⅸ/Ⅹ-bp.