Ciliature Cytoskeleton and Microtubulin of Resting Cyst and Vegetative Cell in Euplotes encysticus

By using optical and transmission electron microscopy,the orientation pattern of ciliature cytoskeleton during resting period in Euplotes encysticus was demonstrated,and the location of α- and β-tubulin in cells under two different physiological states was compared by immunofluorescence.In the resting cyst,nearly all of ciliary shafts were degenerated;kinetosomes of adoral zone of membranelles and ventral cirri were contracted into cytoplasm;part of kinetosomes of frontoventral cirri were disintegrated and disappeared;microtubule layers were preserved beneath the pellicle.It was showed by immunofluorescence that the kinetosomes of dorsal cilia were fully preserved according to the original pattern;after encystment,a slight portion of microtubulin scattered in cytoplasm.On the basis of these experimental results,three destinies of the microtubulin in Euplotes encysticus have been proposed:1)be located and gradually digested within autophagic vacuole,2)be located in cortex and cytoplasm in a form of "microtubulin storehouse",or 3)be preserved in remained kinetosomes.Moreover,the cortical cirri-base-associated fiber found in the past research was not marked in this experiment,which implied that the fiber system does not belong to microtubule system.